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Anti-MAP2K5 Rabbit Monoclonal Antibody #2320

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Flow cytometric analysis of MEK5 expression in HepG2 cells using MEK5 antibody (Cat#2320, 1:2,000). Green, isotype control; red, MEK5.
Immunocytochemical staining of HepG2 cells with anti-MEK5 antibody (Cat#2320, 1:1,000). Nuclei were stained blue with DAPI; MEK5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MEK5 antibody (Cat#2320). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEK5 antibody (Cat#2320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    MAP2K5
  • 货号：
    2320
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-MAP2K5 Rabbit Monoclonal Antibody
Aliases Mitogen-Activated Protein Kinase Kinase 5; MEK5; Dual Specificity Mitogen-Activated Protein Kinase Kinase 5; MAP Kinase Kinase 5; MAPK/ERK Kinase 5; HsT17454; MAPKK5; PRKMK5; EC 2.7.12.2; MAPKK 5; MEK 5; MAP Kinase Kinase MEK5b; MKK5
Background

Gene Name: MAP2K5

NCBI Gene Entry: 5607

UniProt Entry: Q13163
Application Information

Molecular Weight: Predicted, 50 kDa; observed, 49 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB295

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human MEK5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MEK5 expression in HepG2 cells using MEK5 antibody (Cat#2320, 1:2,000). Green, isotype control; red, MEK5.
  • Immunocytochemical staining of HepG2 cells with anti-MEK5 antibody (Cat#2320, 1:1,000). Nuclei were stained blue with DAPI; MEK5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-MEK5 antibody (Cat#2320). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEK5 antibody (Cat#2320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    MAP2K5
  • 货号：
    2320
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of MEK5 expression in HepG2 cells using MEK5 antibody (Cat#2320, 1:2,000). Green, isotype control; red, MEK5.
Immunocytochemical staining of HepG2 cells with anti-MEK5 antibody (Cat#2320, 1:1,000). Nuclei were stained blue with DAPI; MEK5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MEK5 antibody (Cat#2320). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MEK5 antibody (Cat#2320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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