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Anti-RAP1A + RAP1B Rabbit Monoclonal Antibody #2477

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Flow cytometric analysis of RAP1A + RAP1B expression in HeLa cells using RAP1A + RAP1B antibody (Cat#2477, 1:2,000). Green, isotype control; red, RAP1A + RAP1B.
Immunocytochemical staining of Hela cells with anti-RAP1A + RAP1B antibody (Cat#2477, 1:1,000). Nuclei were stained blue with DAPI; RAP1A + RAP1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-RAP1A + RAP1B antibody (Cat#2477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAP1A + RAP1B antibody (Cat#2477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    RAP1B/ RAP1A
  • 货号:
    2477
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-RAP1A + RAP1B Rabbit Monoclonal Antibody
Aliases RAP1B, Member Of RAS Oncogene Family; K-REV; RAL1B; GTP-Binding Protein Smg P21B; Ras-Related Protein Rap-1b; DKFZp586H0723; Ras Family Small GTP Binding Protein RAP1B; RAS-Related Protein RAP1B; Small GTP Binding Protein; EC 3.6.5.2; RAP1A, Member Of RAS Oncogene Family; KREV-1; SMGP21; GTP-Binding Protein Smg P21A; Ras-Related Protein Rap-1A; Ras-Related Protein Krev-1; C21KG; G-22K; KREV1; EC 3.6.5.2; RAP1
Background

Gene Name: RAP1B/ RAP1A

NCBI Gene Entry: 5908/5906

UniProt Entry: P61224/P62834

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1030

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human RAP1A + RAP1B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RAP1A + RAP1B expression in HeLa cells using RAP1A + RAP1B antibody (Cat#2477, 1:2,000). Green, isotype control; red, RAP1A + RAP1B.
  • Immunocytochemical staining of Hela cells with anti-RAP1A + RAP1B antibody (Cat#2477, 1:1,000). Nuclei were stained blue with DAPI; RAP1A + RAP1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-RAP1A + RAP1B antibody (Cat#2477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAP1A + RAP1B antibody (Cat#2477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of RAP1A + RAP1B expression in HeLa cells using RAP1A + RAP1B antibody (Cat#2477, 1:2,000). Green, isotype control; red, RAP1A + RAP1B.
Immunocytochemical staining of Hela cells with anti-RAP1A + RAP1B antibody (Cat#2477, 1:1,000). Nuclei were stained blue with DAPI; RAP1A + RAP1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-RAP1A + RAP1B antibody (Cat#2477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAP1A + RAP1B antibody (Cat#2477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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