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Anti-Pleiotrophin Rabbit Monoclonal Antibody #2494

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Immunocytochemical staining of C2C12 cells with anti-Pleiotrophin antibody (Cat#2494, 1:1,000). Nuclei were stained blue with DAPI; Pleiotrophin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-Pleiotrophin antibody (Cat#2494). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Pleiotrophin antibody (Cat#2494, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PTN
货号：

2494
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Pleiotrophin Rabbit Monoclonal Antibody
Aliases	Pleiotrophin; HBNF; HBGF8; NEGF1; Heparin-Binding Neurite Outgrowth-Promoting Factor 1; Heparin-Binding Growth-Associated Molecule; Heparin-Binding Growth Factor 8; Heparin-Binding Brain Mitogen; Osteoblast-Specific Factor 1; HB-GAM; HBGF-8; HBNF-1; OSF-1; HBBM; Pleiotrophin (Heparin Binding Growth Factor 8, Neurite Growth-Promoting Factor 1); Heparin-Binding Neurite Outgrowth Promoting Factor; Heparin-Binding Neurite Outgrowth-Promoting Factor; Neurite Growth-Promoting Factor 1; Heparin Affin Regulatory Protein; Heparin Binding Growth Factor 8; HBNF1; HARP
Background	Gene Name: PTN NCBI Gene Entry: 5764 UniProt Entry: P21246
Application Information	Molecular Weight: Predicted, 19 kDa; observed, 18 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1110 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Pleiotrophin
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Immunocytochemical staining of C2C12 cells with anti-Pleiotrophin antibody (Cat#2494, 1:1,000). Nuclei were stained blue with DAPI; Pleiotrophin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Pleiotrophin antibody (Cat#2494). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Pleiotrophin antibody (Cat#2494, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PTN
货号：

2494
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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