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Anti-Lamin A/C Rabbit Monoclonal Antibody #3050

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Flow cytometric analysis of Lamin A/C expression in C2C12 cells using anti-Lamin A/C antibody (Cat#3050, 1:2,000). Green, isotype control; red, Lamin A/C.
Immunocytochemical staining of C2C12 cells with anti-Lamin A/C antibody (Cat#3050, 1:1,000) . Nuclei were stained blue with DAPI; Lamin A/C was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Lamin A/C antibody (Cat#3050). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lamin A/C antibody (Cat#3050, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    LMNA
  • 货号:
    3050
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Lamin A/C Rabbit Monoclonal Antibody
Aliases LMNA; Lamin A/C; HGPS; MADA; LMN1; Mandibuloacral Dysplasia Type A; Lamin A/C-Like 1; Prelamin-A/C; Progerin; LGMD1B; CMD1A; LMNL1; PRO1; Limb Girdle Muscular Dystrophy 1B (Autosomal Dominant); Cardiomyopathy, Dilated 1A (Autosomal Dominant); Epididymis Secretory Sperm Binding Protein; Progeria 1 (Hutchinson-Gilford Type); Renal Carcinoma Antigen NY-REN-32; 70 KDa Lamin; Lamin C; CMT2B1; CDCD1; FPLD2; Lamin; CDDC; EMD2; FPLD; LDP1; LMNC; FPL; IDC; LFP
Background

Gene Name: LMNA

NCBI Gene Entry: 4000

UniProt Entry: P02545

Application Information

Molecular Weight: Predicted, 74 kDa; observed, 40-74 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3815

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Lamin A/C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Lamin A/C expression in C2C12 cells using anti-Lamin A/C antibody (Cat#3050, 1:2,000). Green, isotype control; red, Lamin A/C.
  • Immunocytochemical staining of C2C12 cells with anti-Lamin A/C antibody (Cat#3050, 1:1,000) . Nuclei were stained blue with DAPI; Lamin A/C was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Lamin A/C antibody (Cat#3050). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lamin A/C antibody (Cat#3050, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Lamin A/C expression in C2C12 cells using anti-Lamin A/C antibody (Cat#3050, 1:2,000). Green, isotype control; red, Lamin A/C.
Immunocytochemical staining of C2C12 cells with anti-Lamin A/C antibody (Cat#3050, 1:1,000) . Nuclei were stained blue with DAPI; Lamin A/C was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Lamin A/C antibody (Cat#3050). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lamin A/C antibody (Cat#3050, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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