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Anti-SRSF1 Rabbit Monoclonal Antibody #2656

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Flow cytometric analysis of SRSF1 expression in HepG2 cells using anti-SRSF1 antibody (Cat#2656, 1:2,000). Green, isotype control; red, SRSF1.

Immunocytochemical staining of HepG2 cells with anti-SRSF1 antibody (Cat#2656, 1:1,000). Nuclei were stained blue with DAPI; SRSF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-SRSF1 antibody (Cat#2656). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SRSF1 antibody (Cat#2656, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SRSF1
货号：

2656
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-SRSF1 Rabbit Monoclonal Antibody
Aliases	SRSF1; Serine And Arginine Rich Splicing Factor 1; ASF; SF2; Pre-MRNA-Splicing Factor SF2, P33 Subunit; Serine/Arginine-Rich Splicing Factor 1; SRp30a; SF2p33; SFRS1; Splicing Factor, Arginine/Serine-Rich 1; Alternative-Splicing Factor 1; SR Splicing Factor 1; Splicing Factor 2; MGC5228; Splicing Factor, Arginine/Serine-Rich, 30-KD, A; Alternate Splicing Factor; NEDFBA; SF2P33; ASF-1
Background	Gene Name: SRSF1 NCBI Gene Entry: 6426 UniProt Entry: Q07955
Application Information	Molecular Weight: Predicted, 28 kDa; observed, 33 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2255 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SF2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of SRSF1 expression in HepG2 cells using anti-SRSF1 antibody (Cat#2656, 1:2,000). Green, isotype control; red, SRSF1.
Immunocytochemical staining of HepG2 cells with anti-SRSF1 antibody (Cat#2656, 1:1,000). Nuclei were stained blue with DAPI; SRSF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SRSF1 antibody (Cat#2656). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SRSF1 antibody (Cat#2656, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SRSF1
货号：

2656
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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