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Anti-KLF Transcription Factor 4 Mouse Monoclonal Antibody #2832

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Immunocytochemical staining of HT-1080 cells with anti-Checkpoint kinase 1 antibody (Cat#2831, 1:1,000). Nuclei were stained blue with DAPI; Checkpoint kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-KLF4 antibody (Cat#2832). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KLF4 antibody (Cat#2832, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    KLF4
  • 货号:
    2832
  • 应用:
    WB, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-KLF Transcription Factor 4 Mouse Monoclonal Antibody
Aliases KLF4; KLF Transcription Factor 4; GKLF; EZF; Epithelial Zinc Finger Protein EZF; Gut-Enriched Krueppel-Like Factor; Kruppel-Like Factor 4 (Gut); Gut Kruppel-Like Factor; Krueppel-Like Factor 4; Kruppel Like Factor 4; Endothelial Kruppel-Like Zinc Finger Protein
Background

Gene Name: KLF4

NCBI Gene Entry: 9314

UniProt Entry: O43474

Application Information

Molecular Weight: Predicted, 55 kDa; observed, 70 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2750

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen Recombinant protein of human KLF4
Isotype Mouse IgG2b kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HT-1080 cells with anti-Checkpoint kinase 1 antibody (Cat#2831, 1:1,000). Nuclei were stained blue with DAPI; Checkpoint kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-KLF4 antibody (Cat#2832). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KLF4 antibody (Cat#2832, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Immunocytochemical staining of HT-1080 cells with anti-Checkpoint kinase 1 antibody (Cat#2831, 1:1,000). Nuclei were stained blue with DAPI; Checkpoint kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-KLF4 antibody (Cat#2832). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KLF4 antibody (Cat#2832, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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