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Anti-Cystatin B Rabbit Monoclonal Antibody #2895

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Flow cytometric analysis of Cystatin B expression in H9c2 cells using anti-Cystatin B antibody (Cat#2895, 1:2,000). Green, isotype control; red, Cystatin B.

Immunocytochemical staining of H9C2 cells with anti-Cystatin B antibody (Cat#2895, 1:1,000) . Nuclei were stained blue with DAPI; Cystatin B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-Cystatin B antibody (Cat#2895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cystatin B antibody (Cat#2895, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CSTB
货号：

2895
应用：

WB, FCM, IC
物种反应性：

R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Cystatin B Rabbit Monoclonal Antibody
Aliases	CSTB; Cystatin B; CST6; STFB; PME; Liver Thiol Proteinase Inhibitor; Cystatin B (Stefin B); Cystatin-B; CPI-B; EPM1; Epididymis Secretory Sperm Binding Protein; Epilepsy, Progressive Myoclonic 1; Stefin B; Stefin-B; EPM1A; ULD
Background	Gene Name: CSTB NCBI Gene Entry: 1476 UniProt Entry: P04080
Application Information	Molecular Weight: Predicted, 11 kDa; observed, 13 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3050 Species Reactivity: Rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Cystatin B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Cystatin B expression in H9c2 cells using anti-Cystatin B antibody (Cat#2895, 1:2,000). Green, isotype control; red, Cystatin B.
Immunocytochemical staining of H9C2 cells with anti-Cystatin B antibody (Cat#2895, 1:1,000) . Nuclei were stained blue with DAPI; Cystatin B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Cystatin B antibody (Cat#2895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cystatin B antibody (Cat#2895, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CSTB
货号：

2895
应用：

WB, FCM, IC
物种反应性：

R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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