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Anti-MutS Homolog 6 Rabbit Monoclonal Antibody #2912

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Flow cytometric analysis of MutS homolog 6 expression in HepG2 cells using anti-MutS homolog 6 antibody (Cat#2912, 1:2,000). Green, isotype control; red, MutS homolog 6.
Immunocytochemical staining of HepG2 cells with anti-MutS homolog 6 antibody (Cat#2912, 1:1,000) . Nuclei were stained blue with DAPI; MutS homolog 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MutS homolog 6 antibody (Cat#2912). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MutS homolog 6 antibody (Cat#2912, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    MSH6
  • 货号:
    2912
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-MutS Homolog 6 Rabbit Monoclonal Antibody
Aliases MSH6; MutS Homolog 6; MSH-6; GTBP; DNA Mismatch Repair Protein Msh6; G/T Mismatch-Binding Protein; MutS-Alpha 160 KDa Subunit; MutS Protein Homolog 6; GTMBP; P160; MutS (E. Coli) Homolog 6; MutS Homolog 6 (E. Coli); Sperm-Associated Protein; MutS-Like Protein 6; HNPCC5; LYNCH5; MMRCS3; HMSH6; HSAP
Background

Gene Name: MSH6

NCBI Gene Entry: 2956

UniProt Entry: P52701

Application Information

Molecular Weight: Predicted, 153 kDa; observed, 163 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3135

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human MSH6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MutS homolog 6 expression in HepG2 cells using anti-MutS homolog 6 antibody (Cat#2912, 1:2,000). Green, isotype control; red, MutS homolog 6.
  • Immunocytochemical staining of HepG2 cells with anti-MutS homolog 6 antibody (Cat#2912, 1:1,000) . Nuclei were stained blue with DAPI; MutS homolog 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-MutS homolog 6 antibody (Cat#2912). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MutS homolog 6 antibody (Cat#2912, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of MutS homolog 6 expression in HepG2 cells using anti-MutS homolog 6 antibody (Cat#2912, 1:2,000). Green, isotype control; red, MutS homolog 6.
Immunocytochemical staining of HepG2 cells with anti-MutS homolog 6 antibody (Cat#2912, 1:1,000) . Nuclei were stained blue with DAPI; MutS homolog 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MutS homolog 6 antibody (Cat#2912). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MutS homolog 6 antibody (Cat#2912, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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