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Anti-ACADM Rabbit Monoclonal Antibody #2917

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Flow cytometric analysis of ACADM expression in HepG2 cells using anti-ACADM antibody (Cat#2917, 1:2,000). Green, isotype control; red, ACADM.
Immunocytochemical staining of HepG2 cells with anti-ACADM alpha (Ser51) antibody (Cat#2917, 1:1,000). Nuclei were stained blue with DAPI; ACADM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ACADM antibody (Cat#2917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACADM antibody (Cat#2917, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    ACADM
  • 货号:
    2917
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-ACADM Rabbit Monoclonal Antibody
Aliases ACADM; Acyl-CoA Dehydrogenase Medium Chain; MCADH; MCAD; ACAD1; Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; Acyl-Coenzyme A Dehydrogenase, C-4 To C-12 Straight Chain; Medium-Chain Acyl-CoA Dehydrogenase; Acyl-CoA Dehydrogenase, C-4 To C-12 Straight Chain; Medium Chain Acyl-CoA Dehydrogenase; Testicular Tissue Protein Li 7; EC 1.3.99.3; EC 1.3.8.7; EC 1.3.99
Background

Gene Name: ACADM

NCBI Gene Entry: 34

UniProt Entry: P11310

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 46 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3160

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human ACADM
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ACADM expression in HepG2 cells using anti-ACADM antibody (Cat#2917, 1:2,000). Green, isotype control; red, ACADM.
  • Immunocytochemical staining of HepG2 cells with anti-ACADM alpha (Ser51) antibody (Cat#2917, 1:1,000). Nuclei were stained blue with DAPI; ACADM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-ACADM antibody (Cat#2917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACADM antibody (Cat#2917, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of ACADM expression in HepG2 cells using anti-ACADM antibody (Cat#2917, 1:2,000). Green, isotype control; red, ACADM.
Immunocytochemical staining of HepG2 cells with anti-ACADM alpha (Ser51) antibody (Cat#2917, 1:1,000). Nuclei were stained blue with DAPI; ACADM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ACADM antibody (Cat#2917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACADM antibody (Cat#2917, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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