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KO-Validated Anti-GAPDH Rabbit Monoclonal Antibody #71169

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Western blotting analysis using anti-GAPDH antibody (Cat#71169). GAPDH expression in wild type (WT) and GAPDH knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GAPDH antibody (Cat#71169, 1:100,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-GAPDH antibody (Cat#71169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GAPDH antibody (Cat#71169, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    GAPDH
  • 货号:
    71169
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
50 μL,100 μL
价格:
¥ 600.00,¥ 1,000.00
Information
Product Name KO-Validated Anti-GAPDH Rabbit Monoclonal Antibody
Aliases GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein; OCAS, P38 Component ; HEL-S-162eP; EC 2.6.99.-; EC 1.2.1; G3PD
Background

Gene Name: GAPDH

NCBI Gene Entry: 2597

UniProt Entry: P04406

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB620

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human GAPDH
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:2,000-1:10,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-GAPDH antibody (Cat#71169). GAPDH expression in wild type (WT) and GAPDH knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GAPDH antibody (Cat#71169, 1:100,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-GAPDH antibody (Cat#71169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GAPDH antibody (Cat#71169, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Western blotting analysis using anti-GAPDH antibody (Cat#71169). GAPDH expression in wild type (WT) and GAPDH knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GAPDH antibody (Cat#71169, 1:100,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-GAPDH antibody (Cat#71169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GAPDH antibody (Cat#71169, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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