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Anti-DOT1L Rabbit Monoclonal Antibody #2919

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Flow cytometric analysis of DOT1 like histone lysine methyltransferase expression in HepG2 cells using anti-DOT1 like histone lysine methyltransferase antibody (Cat#2919, 1:2,000). Green, isotype control; red, DOT1 like histone lysine methyltransferase.

Immunocytochemical staining of HepG2 cells with anti-DOT1 like histone lysine methyltransferase antibody (Cat#2919, 1:1,000). Nuclei were stained blue with DAPI; DOT1 like histone lysine methyltransferase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-DOT1 like histone lysine methyltransferase antibody (Cat#2919). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DOT1 like histone lysine methyltransferase antibody (Cat#2919, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

DOT1L
货号：

2919
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-DOT1L Rabbit Monoclonal Antibody
Aliases	DOT1L; DOT1 Like Histone Lysine Methyltransferase; KMT4; KIAA1814; Histone-Lysine N-Methyltransferase, H3 Lysine-79 Specific; Histone H3-K79 Methyltransferase; Histone Methyltransferase DOT1L; Lysine N-Methyltransferase 4; DOT1-Like Protein; H3-K79-HMTas; DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae); DOT1 Like Histone H3K79 Methyltransferase; DOT1-Like, Histone H3 Methyltransferase; DOT1-Like Histone Methyltransferase; EC 2.1.1.360
Background	Gene Name: DOT1L NCBI Gene Entry: 84444 UniProt Entry: Q8TEK3
Application Information	Molecular Weight: Predicted, 165 kDa; observed, 155 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3170 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human KMT4 / Dot1L
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of DOT1 like histone lysine methyltransferase expression in HepG2 cells using anti-DOT1 like histone lysine methyltransferase antibody (Cat#2919, 1:2,000). Green, isotype control; red, DOT1 like histone lysine methyltransferase.
Immunocytochemical staining of HepG2 cells with anti-DOT1 like histone lysine methyltransferase antibody (Cat#2919, 1:1,000). Nuclei were stained blue with DAPI; DOT1 like histone lysine methyltransferase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-DOT1 like histone lysine methyltransferase antibody (Cat#2919). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DOT1 like histone lysine methyltransferase antibody (Cat#2919, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

DOT1L
货号：

2919
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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