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Anti-NR4A1 Rabbit Monoclonal Antibody #2971

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Flow cytometric analysis of NR4A1 expression in HepG2 cells using anti-NR4A1 antibody (Cat#2971, 1:2,000). Green, isotype control; red, NR4A1.
Immunocytochemical staining of HepG2 cells with anti-NR4A1 antibody (Cat#2971, 1:1,000). Nuclei were stained blue with DAPI; NR4A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NR4A1 antibody (Cat#2971). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NR4A1 antibody (Cat#2971, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    NR4A1
  • 货号:
    2971
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-NR4A1 Rabbit Monoclonal Antibody
Aliases NR4A1; Nuclear Receptor Subfamily 4 Group A Member 1; NAK-1; NGFIB; NUR77; GFRP1; TR3; N10; HMR; Nuclear Receptor Subfamily 4immunitygroup A Member 1; Nuclear Hormone Receptor NUR/77; Early Response Protein NAK1; Orphan Nuclear Receptor HMR; Orphan Nuclear Receptor TR3; Testicular Receptor 3; ST-59; Nuclear Receptor Subfamily 4, Group A, Member 1; Growth Factor-Inducible Nuclear Protein N10; Steroid Receptor TR3; TR3 Orphan Receptor; Hormone Receptor; Nur77; NP10; NAK1
Background

Gene Name: NR4A1

NCBI Gene Entry: 3164

UniProt Entry: P22736

Application Information

Molecular Weight: Predicted, 64 kDa; observed, 64 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3420

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human NUR77
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NR4A1 expression in HepG2 cells using anti-NR4A1 antibody (Cat#2971, 1:2,000). Green, isotype control; red, NR4A1.
  • Immunocytochemical staining of HepG2 cells with anti-NR4A1 antibody (Cat#2971, 1:1,000). Nuclei were stained blue with DAPI; NR4A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-NR4A1 antibody (Cat#2971). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NR4A1 antibody (Cat#2971, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of NR4A1 expression in HepG2 cells using anti-NR4A1 antibody (Cat#2971, 1:2,000). Green, isotype control; red, NR4A1.
Immunocytochemical staining of HepG2 cells with anti-NR4A1 antibody (Cat#2971, 1:1,000). Nuclei were stained blue with DAPI; NR4A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NR4A1 antibody (Cat#2971). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NR4A1 antibody (Cat#2971, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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