Flow cytometric analysis of Annexin A4 expression in C2C12 cells using anti-Annexin A4 antibody (Cat#63252, 1:2,000). Green, isotype control; red, Annexin A4.
Immunocytochemical staining of C2C12 cells with anti-Annexin A4 antibody (Cat#63252, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-annexin A4 antibody (Cat#63252). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A4 antibody (Cat#63252, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-annexin A4 antibody (Cat#63252). Annexin A4 expression in wild-type (WT) and annexin A4 (ANXA4) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A4 antibody (Cat#63252, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
