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Anti-Phospho-TrkA/B (Tyr490/Tyr516) Rabbit Monoclonal Antibody #3193

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Flow cytometric analysis of Phospho-TrkA/B (Tyr490/Tyr516) expression in HepG2 cells using anti-Phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193, 1:2,000). Green, isotype control; red, Phospho-TrkA/B (Tyr490/Tyr516).
Immunocytochemical staining of HepG2 cells with anti-phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-TrkA/B (Tyr490/Tyr516) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    NTRK1/2
  • 货号：
    3193
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Phospho-TrkA/B (Tyr490/Tyr516) Rabbit Monoclonal Antibody
Aliases NTRK1; Neurotrophic Receptor Tyrosine Kinase 1; TRKA; TRK; MTC; High Affinity Nerve Growth Factor Receptor; Neurotrophic Tyrosine Kinase, Receptor, Type 1; TRK1-Transforming Tyrosine Kinase Protein; Tropomyosin Receptor Kinase A; Tropomyosin-Related Kinase A; Tyrosine Kinase Receptor A; EC 2.7.10.1; P140-TrkA; Gp140trk; Trk-A; Neurotrophic Tyrosine Kinase Receptor Type 1; Tyrosine Kinase Receptor; Oncogene TRK; EC 2.7.10; TRK1; NTRK2; Neurotrophic Receptor Tyrosine Kinase 2; TRKB; BDNF/NT-3 Growth Factors Receptor; Neurotrophic Tyrosine Kinase Receptor Type 2; Tropomyosin-Related Kinase B; EC 2.7.10.1; GP145-TrkB; Trk-B; Neurotrophic Tyrosine Kinase, Receptor, Type 2; BDNF-Tropomyosine Receptor Kinase B; Tyrosine Kinase Receptor B; TrkB Tyrosine Kinase; EC 2.7.10; EIEE58 ; DEE58; OBHD
Background

Gene Name: NTRK1/2

NCBI Gene Entry: 4914/4915

UniProt Entry: P04629/Q16620
Application Information

Molecular Weight: Predicted, 92 kDa; observed, 180 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4455

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human Phospho-TrkA/B (Tyr490/Tyr516)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-TrkA/B (Tyr490/Tyr516) expression in HepG2 cells using anti-Phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193, 1:2,000). Green, isotype control; red, Phospho-TrkA/B (Tyr490/Tyr516).
  • Immunocytochemical staining of HepG2 cells with anti-phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-TrkA/B (Tyr490/Tyr516) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    NTRK1/2
  • 货号：
    3193
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-TrkA/B (Tyr490/Tyr516) expression in HepG2 cells using anti-Phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193, 1:2,000). Green, isotype control; red, Phospho-TrkA/B (Tyr490/Tyr516).
Immunocytochemical staining of HepG2 cells with anti-phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-TrkA/B (Tyr490/Tyr516) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-TrkA/B (Tyr490/Tyr516) antibody (Cat#3193, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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