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Anti-Protein Kinase C Gamma Rabbit Monoclonal Antibody #3202

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Flow cytometric analysis of PKC gamma expression in HepG2 cells using anti-PKC gamma antibody (Cat#3202, 1:2,000). Green, isotype control; red, PKC gamma.
Immunocytochemical staining of HepG2 cells with anti-PKC gamma antibody(Cat#3202, 1:1,000) . Nuclei were stained blue with DAPI; PKC gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PKC gamma antibody (Cat#3202). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKC gamma antibody (Cat#3202, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    PRKCG
  • 货号:
    3202
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Protein Kinase C Gamma Rabbit Monoclonal Antibody
Aliases PRKCG; Protein Kinase C Gamma; PKC-Gamma; PKCC; PKCG; Protein Kinase C Gamma Type; EC 2.7.11.13; MGC57564;SCA14; Protein Kinase C, Gamma; EC 2.7.11; PKCgamma; PKCI(3); PKCγ
Background

Gene Name: PRKCG

NCBI Gene Entry: 5582

UniProt Entry: P05129

Application Information

Molecular Weight: Predicted, 78 kDa; observed, 78 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4495

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human PKC
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PKC gamma expression in HepG2 cells using anti-PKC gamma antibody (Cat#3202, 1:2,000). Green, isotype control; red, PKC gamma.
  • Immunocytochemical staining of HepG2 cells with anti-PKC gamma antibody(Cat#3202, 1:1,000) . Nuclei were stained blue with DAPI; PKC gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-PKC gamma antibody (Cat#3202). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKC gamma antibody (Cat#3202, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of PKC gamma expression in HepG2 cells using anti-PKC gamma antibody (Cat#3202, 1:2,000). Green, isotype control; red, PKC gamma.
Immunocytochemical staining of HepG2 cells with anti-PKC gamma antibody(Cat#3202, 1:1,000) . Nuclei were stained blue with DAPI; PKC gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PKC gamma antibody (Cat#3202). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKC gamma antibody (Cat#3202, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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