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Anti-Phospho-PKR (T451) Rabbit Monoclonal Antibody #4632

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Flow cytometric analysis of Phospho-Phospho-PKR (T451) expression in HT-1080 cells using anti-Phospho-Phospho-PKR (T451) antibody (Cat#4632, 1:2,000). Green, isotype control; red, Phospho-Phospho-PKR (T451).
Immunocytochemical staining of HT-1080 cells with anti-Phospho-Phospho-PKR (T451)  antibody (Cat#4632, 1:1,000) . Nuclei were stained blue with DAPI;Phospho-Phospho-PKR (T451)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-phospho PKR(T451) antibody (Cat#4632). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-phospho PKR(T451) antibody (Cat#4632, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    EIF2AK2
  • 货号：
    4632
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Phospho-PKR (T451) Rabbit Monoclonal Antibody
Aliases EIF2AK2; Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2; PKR; Protein Kinase R; PPP1R83; PRKR; Protein Kinase, Interferon-Inducible Double Stranded RNA Dependent; Interferon-Induced, Double-Stranded RNA-Activated Protein Kinase; Protein Phosphatase 1, Regulatory Subunit 83; Tyrosine-Protein Kinase EIF2AK2; Protein Kinase RNA-Activated; Protein Kinase RNA-Regulated; P1/EIF-2A Protein Kinase; EIF-2A Protein Kinase 2; P68 Kinase; Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2; Interferon-Inducible RNA-Dependent Protein Kinase; Double Stranded RNA Activated Protein Kinase; Interferon-Inducible ElF2alpha Kinase; EC 2.7.11.1; EC 2.7.10.2; EIF2AK1; LEUDEN; DYT33
Background

Gene Name: EIF2AK2

NCBI Gene Entry: 5610

UniProt Entry: P19525
Application Information

Molecular Weight: Predicted, 62 kDa; observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12170

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human eIF2A K2 around the phosphorylation site of T451
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-Phospho-PKR (T451) expression in HT-1080 cells using anti-Phospho-Phospho-PKR (T451) antibody (Cat#4632, 1:2,000). Green, isotype control; red, Phospho-Phospho-PKR (T451).
  • Immunocytochemical staining of HT-1080 cells with anti-Phospho-Phospho-PKR (T451)  antibody (Cat#4632, 1:1,000) . Nuclei were stained blue with DAPI;Phospho-Phospho-PKR (T451)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-phospho-phospho PKR(T451) antibody (Cat#4632). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-phospho PKR(T451) antibody (Cat#4632, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    EIF2AK2
  • 货号：
    4632
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-Phospho-PKR (T451) expression in HT-1080 cells using anti-Phospho-Phospho-PKR (T451) antibody (Cat#4632, 1:2,000). Green, isotype control; red, Phospho-Phospho-PKR (T451).
Immunocytochemical staining of HT-1080 cells with anti-Phospho-Phospho-PKR (T451)  antibody (Cat#4632, 1:1,000) . Nuclei were stained blue with DAPI;Phospho-Phospho-PKR (T451)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-phospho PKR(T451) antibody (Cat#4632). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-phospho PKR(T451) antibody (Cat#4632, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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