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Anti-EIF3B Rabbit Monoclonal Antibody #3211

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Flow cytometric analysis of EIF3B expression in HepG2 cells using anti-EIF3B antibody (Cat#3211, 1:2,000). Green, isotype control; red, EIF3B.

Immunocytochemical staining of HepG2 cells with anti-EIF3B antibody (Cat#3211, 1:1,000). Nuclei were stained blue with DAPI; EIF3B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-EIF3B antibody (Cat#3211). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF3B antibody (Cat#3211, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). EIF3B, eukaryotic translation initiation factor 3 subunit B.

基因名：

EIF3B
货号：

3211
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-EIF3B Rabbit Monoclonal Antibody
Aliases	EIF3B; Eukaryotic Translation Initiation Factor 3 Subunit B; EIF3S9; PRT1; Eukaryotic Translation Initiation Factor 3, Subunit 9 Eta, 116kDa; Eukaryotic Translation Initiation Factor 3 Subunit 9; Protein Synthesis 1; Prt1 Homolog; Eukaryotic Translation Initiation Factor 3, Subunit 9 (Eta, 116kD); Eukaryotic Translation Initiation Factor 3, Subunit B; EIF3-P110; EIF3-P116; EIF-3-Eta; EIF3 P110; EIF3 P116; EIF3-ETA; EIF3b; HPrt1
Background	Gene Name: EIF3B NCBI Gene Entry: 8662 UniProt Entry: P55884
Application Information	Molecular Weight: Predicted, 92 kDa; observed, 117 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4540 Species Reactivity: Human Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human eIF3B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of EIF3B expression in HepG2 cells using anti-EIF3B antibody (Cat#3211, 1:2,000). Green, isotype control; red, EIF3B.
Immunocytochemical staining of HepG2 cells with anti-EIF3B antibody (Cat#3211, 1:1,000). Nuclei were stained blue with DAPI; EIF3B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-EIF3B antibody (Cat#3211). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF3B antibody (Cat#3211, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). EIF3B, eukaryotic translation initiation factor 3 subunit B.

Citation(0)

基因名：

EIF3B
货号：

3211
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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