Anti-Phospho-Vimentin (Ser72) Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-Vimentin (Ser72) Rabbit Monoclonal Antibody #3240

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Flow cytometric analysis of Phospho-Vimentin (Ser72) expression in HT-1080 cells using anti-Phospho-Vimentin (Ser72) antibody (Cat#3240, 1:2,000). Green, isotype control; red, Phospho-Vimentin (Ser72).

Immunocytochemical staining of HT-1080 cells with anti-Phospho-Vimentin (Ser72) antibody(Cat#3240, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-Vimentin (Ser72) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-phospho-vimentin (Ser72) antibody (Cat#3240). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-vimentin (Ser72) antibody (Cat#3240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using anti-phospho-vimentin (Ser72) antibody (Cat#3240, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

基因名：

VIM
货号：

3240
应用：

WB, FCM, IC, IHC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Phospho-Vimentin (Ser72) Rabbit Monoclonal Antibody
Aliases	VIM; Vimentin; Epididymis Secretory Sperm Binding Protein 3
Background	Gene Name: VIM NCBI Gene Entry: 7431 UniProt Entry: P08670
Application Information	Molecular Weight: Predicted, 54 kDa; observed, 54 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4665 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen	A synthesized peptide derived from human Phospho-Vimentin (Ser72)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000 Immunohistochemistry (IHC): 1:50-1:100
Note	This product is for research use only.

Picture

Flow cytometric analysis of Phospho-Vimentin (Ser72) expression in HT-1080 cells using anti-Phospho-Vimentin (Ser72) antibody (Cat#3240, 1:2,000). Green, isotype control; red, Phospho-Vimentin (Ser72).
Immunocytochemical staining of HT-1080 cells with anti-Phospho-Vimentin (Ser72) antibody(Cat#3240, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-Vimentin (Ser72) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-vimentin (Ser72) antibody (Cat#3240). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-vimentin (Ser72) antibody (Cat#3240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using anti-phospho-vimentin (Ser72) antibody (Cat#3240, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Citation(0)

基因名：

VIM
货号：

3240
应用：

WB, FCM, IC, IHC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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