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Anti-RCHY1 Rabbit Monoclonal Antibody #3334

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Flow cytometric analysis of RCHY1 expression in HAP-1 cells using anti-RCHY1 antibody (Cat#3334, 1:2,000). Green, isotype control; red, RCHY1.
Immunocytochemical staining of HAP-1 with anti-RCHY1 antibody (Cat#3334, 1:1,000) . Nuclei were stained blue with DAPI; RCHY1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-RCHY1 antibody (Cat#3334). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RCHY1 antibody (Cat#3334, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    RCHY1
  • 货号:
    3334
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-RCHY1 Rabbit Monoclonal Antibody
Aliases RCHY1; Ring Finger And CHY Zinc Finger Domain Containing 1; RNF199; ZNF363; CHIMP; ARNIP; PIRH2; PRO1996; ZCHY; RING Finger And CHY Zinc Finger Domain-Containing Protein 1; Androgen-Receptor N-Terminal-Interacting Protein; P53-Induced Protein With A RING-H2 Domain; RING-Type E3 Ubiquitin Transferase RCHY1; E3 Ubiquitin-Protein Ligase Pirh2; RING Finger Protein 199; Zinc Finger Protein 363; Zinc Finger, CHY-Type; DKFZp586C1620; Ring Finger And CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase; Androgen Receptor N-Terminal-Interacting Protein; CH-Rich Interacting Match With PLAG1; CH-Rich-Interacting Match With PLAG1; P53-Induced RING-H2 Protein; EC 2.3.2.27; HPirh2
Background

Gene Name: RCHY1

NCBI Gene Entry: 25898

UniProt Entry: Q96PM5

Application Information

Molecular Weight: Predicted, 30 kDa; observed, 26 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5165

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human RCHY1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RCHY1 expression in HAP-1 cells using anti-RCHY1 antibody (Cat#3334, 1:2,000). Green, isotype control; red, RCHY1.
  • Immunocytochemical staining of HAP-1 with anti-RCHY1 antibody (Cat#3334, 1:1,000) . Nuclei were stained blue with DAPI; RCHY1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-RCHY1 antibody (Cat#3334). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RCHY1 antibody (Cat#3334, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of RCHY1 expression in HAP-1 cells using anti-RCHY1 antibody (Cat#3334, 1:2,000). Green, isotype control; red, RCHY1.
Immunocytochemical staining of HAP-1 with anti-RCHY1 antibody (Cat#3334, 1:1,000) . Nuclei were stained blue with DAPI; RCHY1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-RCHY1 antibody (Cat#3334). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RCHY1 antibody (Cat#3334, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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