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Anti-RAD51 Paralog D Rabbit Monoclonal Antibody #3336

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Flow cytometric analysis of RAD51D expression in HeLa cells using anti-RAD51D antibody (Cat#3336, 1:2,000). Green, isotype control; red, RAD51D.
Immunocytochemical staining of Hela cells with RAD51D antibody (Cat#3336, 1:1,000). Nuclei were stained blue with DAPI; RAD51D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RAD51D antibody (Cat#3336). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD51D antibody (Cat#3336, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    RAD51D
  • 货号:
    3336
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-RAD51 Paralog D Rabbit Monoclonal Antibody
Aliases RAD51D; RAD51 Paralog D; R51H3; DNA Repair Protein RAD51 Homolog 4; RAD51L3; Trad; Recombination Repair Protein; RAD51-Like Protein 3; RAD51 Homolog D; HsTRAD; RAD51 Homolog D (S. Cerevisiae); RAD51 (S. Cerevisiae)-Like 3; RAD51-Like 3 (S. Cerevisiae); BROVCA4; TRAD
Background

Gene Name: RAD51D

NCBI Gene Entry: 5892

UniProt Entry: O75771

Application Information

Molecular Weight: Predicted, 35 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5175

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Rad51D
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
IImmunohistochemistry (IHC): 1:100-1:1,000
Flow Cytometry (FCM) : 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RAD51D expression in HeLa cells using anti-RAD51D antibody (Cat#3336, 1:2,000). Green, isotype control; red, RAD51D.
  • Immunocytochemical staining of Hela cells with RAD51D antibody (Cat#3336, 1:1,000). Nuclei were stained blue with DAPI; RAD51D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-RAD51D antibody (Cat#3336). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD51D antibody (Cat#3336, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of RAD51D expression in HeLa cells using anti-RAD51D antibody (Cat#3336, 1:2,000). Green, isotype control; red, RAD51D.
Immunocytochemical staining of Hela cells with RAD51D antibody (Cat#3336, 1:1,000). Nuclei were stained blue with DAPI; RAD51D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RAD51D antibody (Cat#3336). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD51D antibody (Cat#3336, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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