Flow cytometric analysis of DNAJA2 expression in C2C12 cells using anti-DNAJA2 antibody (Cat#63838, 1:1,000). Green, isotype control; red, DNAJA2.
Immunocytochemical staining of C2C12 cells with anti-DNAJA2 antibody (Cat#63838, 1:1,000) . Nuclei were stained blue with DAPI; DNAJA2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-DNAJA2 antibody (Cat#63838). Total cell lysates (10 μg for C2C12 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNAJA2 antibody (Cat#63838, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-DNAJA2 antibody (Cat#63838). DNAJA2 expression in wild type (WT) and DNAJA2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNAJA2 antibody (Cat#63838, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of DNAJA2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DNAJA2 antibody (Cat#63838, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-DNAJA2 antibody (Cat#63838, 1:1,000), Top panel: wild-type (WT); Bottom panal: DNAJA2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; DNAJA2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
