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Anti-DBF4 Rabbit Monoclonal Antibody #3374

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Flow cytometric analysis of DBF4 expression in HepG2 cells using anti-DBF4 antibody (Cat#3374, 1:2,000). Green, isotype control; red,DBF4.

Immunocytochemical staining of HepG2 cells with anti-DBF4 antibody (Cat#3374, 1:1,000). Nuclei were stained blue with DAPI; DBF4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-DBF4 antibody (Cat#3374). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DBF4 antibody (Cat#3374, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

DBF4
货号：

3374
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-DBF4 Rabbit Monoclonal Antibody
Aliases	DBF4; DBF4-CDC7 Kinase Regulatory Subunit; ZDBF1; DBF4A; ASK; Activator Of S Phase Kinase; Chif; DBF4-Type Zinc Finger-Containing Protein 1; Zinc Finger, DBF-Type Containing 1; Regulatory Subunit Of DDK; Protein DBF4 Homolog A; DBF4 Zinc Finger A; Chiffon Homolog A; DBF4 Zinc Finger; Chiffon Homolog (Drosophila); DBF4 Homolog (S. Cerevisiae); DBF4 Homolog
Background	Gene Name: DBF4 NCBI Gene Entry: 10926 UniProt Entry: Q9UBU7
Application Information	Molecular Weight: Predicted, 77 kDa; observed, 77 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB5370 Species Reactivity: Human Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human DBF4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of DBF4 expression in HepG2 cells using anti-DBF4 antibody (Cat#3374, 1:2,000). Green, isotype control; red,DBF4.
Immunocytochemical staining of HepG2 cells with anti-DBF4 antibody (Cat#3374, 1:1,000). Nuclei were stained blue with DAPI; DBF4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-DBF4 antibody (Cat#3374). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DBF4 antibody (Cat#3374, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

DBF4
货号：

3374
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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