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Anti-FYN Mouse Monoclonal Antibody #3704

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Flow cytometric analysis of FYN expression in HeLa cells using anti-FYN antibody (Cat#3704, 1:2,000). Green, isotype control; red, FYN.

Immunocytochemical staining of HeLa cells with anti-FYN antibody (Cat#3704, 1:1,000) . Nuclei were stained blue with DAPI; FYN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.

Western blotting analysis using anti-FYN antibody (Cat#3704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FYN antibody (Cat#3704, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

FYN
货号：

3704
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-FYN Mouse Monoclonal Antibody
Aliases	FYN; FYN Proto-Oncogene, Src Family Tyrosine Kinase; SLK; SYN; FYN Oncogene Related To SRC, FGR, YES; Tyrosine-Protein Kinase Fyn; Proto-Oncogene C-Fyn; Proto-Oncogene Syn; Src-Like Kinase; EC 2.7.10.2; MGC45350; OKT3-Induced Calcium Influx Regulator; Tyrosine Kinase P59fyn(T); Src/Yes-Related Novel; C-Syn Protooncogene; EC 2.7.10; P59-FYN; P59-Fyn
Background	Gene Name: FYN NCBI Gene Entry: 2534 UniProt Entry: P06241
Application Information	Molecular Weight: Predicted, 61 kDa; observed, 61 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB6995 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human FYN
Isotype	Mouse IgG1 kappa
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of FYN expression in HeLa cells using anti-FYN antibody (Cat#3704, 1:2,000). Green, isotype control; red, FYN.
Immunocytochemical staining of HeLa cells with anti-FYN antibody (Cat#3704, 1:1,000) . Nuclei were stained blue with DAPI; FYN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
Western blotting analysis using anti-FYN antibody (Cat#3704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FYN antibody (Cat#3704, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

FYN
货号：

3704
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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