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Anti-EHMT2 Rabbit Monoclonal Antibody #3931

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Immunocytochemical staining of HepG2 cells with anti-EHMT2 antibody(Cat#3931, 1:1,000) . Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of EHMT2 expression in HepG2 cells using anti-EHMT2 antibody (Cat#3931, 1:2,000). Green, isotype control; red, EHMT2.
Western blotting analysis using anti-EHMT2 antibody (Cat#3931). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EHMT2 antibody (Cat#3931, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    EHMT2
  • 货号:
    3931
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-EHMT2 Rabbit Monoclonal Antibody
Aliases Euchromatic Histone Lysine Methyltransferase 2; KMT1C; G9A; C6orf30; BAT8; Euchromatic Histone-Lysine N-Methyltransferase 2; Histone-Lysine N-Methyltransferase EHMT2; Histone H3-K9 Methyltransferase 3; HLA-B Associated Transcript 8; Lysine N-Methyltransferase 1C; H3-K9-HMTase 3; Em:AF134726.3; NG36/G9a; NG36; Histone-Lysine N-Methyltransferase, H3 Lysine-9 Specific 3; Chromosome 6 Open Reading Frame 30; Ankyrin Repeat-Containing Protein; G9A Histone Methyltransferase; HLA-B-Associated Transcript 8; EC 2.1.1.367; Protein G9a; EC 2.1.1.-; GAT8
Background

Gene Name: EHMT2

NCBI Gene Entry: 10919

UniProt Entry: Q96KQ7

Application Information

Molecular Weight: Predicted, 132 kDa; observed, 160,170 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB8745

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human EHMT2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with anti-EHMT2 antibody(Cat#3931, 1:1,000) . Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Flow cytometric analysis of EHMT2 expression in HepG2 cells using anti-EHMT2 antibody (Cat#3931, 1:2,000). Green, isotype control; red, EHMT2.
  • Western blotting analysis using anti-EHMT2 antibody (Cat#3931). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EHMT2 antibody (Cat#3931, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Immunocytochemical staining of HepG2 cells with anti-EHMT2 antibody(Cat#3931, 1:1,000) . Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of EHMT2 expression in HepG2 cells using anti-EHMT2 antibody (Cat#3931, 1:2,000). Green, isotype control; red, EHMT2.
Western blotting analysis using anti-EHMT2 antibody (Cat#3931). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EHMT2 antibody (Cat#3931, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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