Immunocytochemical staining of HepG2 cells with anti-NELFB antibody (Cat#61643, 1:1,000). Nuclei were stained blue with DAPI; NELFB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NELFB antibody (Cat#61643). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NELFB antibody (Cat#61643, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NELFB antibody (Cat#61643). NELFB expression in wild-type (WT) and NELFB shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NELFB antibody (Cat#61643, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of NELFB knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with anti-NELFB antibody (Cat#61643, 1:2,000) and analyzed using BD flow cytometer.
