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Anti-RAD50 Rabbit Monoclonal Antibody #4352

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Flow cytometric analysis of RAD50 expression in HeLa cells using anti-RAD50 antibody (Cat#4352, 1:2,000). Green, isotype control; red, RAD50.

Immunocytochemical staining of HeLa cells with anti-RAD50 antibody (Cat#4352, 1:1,000). Nuclei were stained blue with DAPI; RAD50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-RAD50 antibody (Cat#4352). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD50 antibody (Cat#4352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

RAD50
货号：

4352
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-RAD50 Rabbit Monoclonal Antibody
Aliases	RAD50; RAD50 Double Strand Break Repair Protein; RAD50 Homolog, Double Strand Break Repair Protein; DNA Repair Protein RAD50; RAD50-2; HRad50; HRAD50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); EC 3.6.3.27; EC 3.6.1.15; EC 3.6.-.-; EC 3.6.3; RAD502; NBSLD
Background	Gene Name: RAD50 NCBI Gene Entry: 10111 UniProt Entry: Q92878
Application Information	Molecular Weight: Predicted, 154 kDa; observed, 154 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10790 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Rad50
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of RAD50 expression in HeLa cells using anti-RAD50 antibody (Cat#4352, 1:2,000). Green, isotype control; red, RAD50.
Immunocytochemical staining of HeLa cells with anti-RAD50 antibody (Cat#4352, 1:1,000). Nuclei were stained blue with DAPI; RAD50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RAD50 antibody (Cat#4352). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD50 antibody (Cat#4352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

RAD50
货号：

4352
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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