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Anti-PFKFB3 Rabbit Monoclonal Antibody #4399

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Flow cytometric analysis of PFKFB3 expression in HT-1080 cells using anti-PFKFB3 antibody (Cat#4399, 1:2,000). Green, isotype control; red, PFKFB3.

Immunocytochemical staining of HT-1080 cells with anti-PFKFB3 antibody (Cat#4399, 1:1,000). Nuclei were stained blue with DAPI; PFKFB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PFKFB3 antibody (Cat#4399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PFKFB3 antibody (Cat#4399, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PFKFB3
货号：

4399
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Citation(0)

Information

Product Name	Anti-PFKFB3 Rabbit Monoclonal Antibody
Aliases	PFKFB3; 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3; 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase Brain/Placenta-Type Isozyme 3; Renal Carcinoma Antigen NY-REN-56; 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3; IPFK-2; Inducible 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase; Fructose-6-Phosphate,2-Kinase/Fructose-2, 6-Bisphosphatase; 6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase; IPFK2; PFK2
Background	Gene Name: PFKFB3 NCBI Gene Entry: 5209 UniProt Entry: Q16875
Application Information	Molecular Weight: Predicted, 60 kDa; observed, 58 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11025 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PFKFB3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PFKFB3 expression in HT-1080 cells using anti-PFKFB3 antibody (Cat#4399, 1:2,000). Green, isotype control; red, PFKFB3.
Immunocytochemical staining of HT-1080 cells with anti-PFKFB3 antibody (Cat#4399, 1:1,000). Nuclei were stained blue with DAPI; PFKFB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PFKFB3 antibody (Cat#4399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PFKFB3 antibody (Cat#4399, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PFKFB3
货号：

4399
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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