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Anti-MLD Rabbit Monoclonal Antibody #4420

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Flow cytometric analysis of MLD expression in HT-1080 cells using anti-MLD antibody (Cat#4420, 1:2,000). Green, isotype control; red, MLD.
Immunocytochemical staining of HT-1080 cells with anti-MLD antibody (Cat#4420, 1:1,000). Nuclei were stained blue with DAPI; MLD was stained magenta
with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-MLD antibody (Cat#4420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MLD antibody (Cat#4420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    DEGS1
  • 货号:
    4420
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-MLD Rabbit Monoclonal Antibody
Aliases DEGS1; Delta 4-Desaturase, Sphingolipid 1; DES1; MLD; DEGS-1; FADS7; Cell Migration-Inducing Gene 15 Protein; Sphingolipid Delta(4)-Desaturase DES1; Sphingolipid Delta(4)-Desaturase 1; Dihydroceramide Desaturase 1; Membrane Lipid Desaturase; Retinol Isomerase; Des-1; Degenerative Spermatocyte Homolog 1, Lipid Desaturase (Drosophila); Degenerative Spermatocyte Homolog 1, Lipid Desaturase; Degenerative Spermatocyte Homolog, Lipid Desaturase; Membrane Fatty Acid (Lipid) Desaturase; Degenerative Spermatocyte Homolog 1; Migration-Inducing Gene 15 Protein; Sphingolipid Delta 4 Desaturase; Dihydroceramide Desaturase-1; EC 1.14.19.17; EC 5.2.1.-; HLD18; MIG15; DES-1; DEGS
Background

Gene Name: DEGS1

NCBI Gene Entry: 8560

UniProt Entry: O15121

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 34 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11130

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human MLD
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MLD expression in HT-1080 cells using anti-MLD antibody (Cat#4420, 1:2,000). Green, isotype control; red, MLD.
  • Immunocytochemical staining of HT-1080 cells with anti-MLD antibody (Cat#4420, 1:1,000). Nuclei were stained blue with DAPI; MLD was stained magenta
    with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-MLD antibody (Cat#4420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MLD antibody (Cat#4420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of MLD expression in HT-1080 cells using anti-MLD antibody (Cat#4420, 1:2,000). Green, isotype control; red, MLD.
Immunocytochemical staining of HT-1080 cells with anti-MLD antibody (Cat#4420, 1:1,000). Nuclei were stained blue with DAPI; MLD was stained magenta
with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-MLD antibody (Cat#4420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MLD antibody (Cat#4420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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