Flow cytometric analysis of sorting nexin 5 expression in HAP-1 cells using anti-sorting nexin 5 antibody (Cat#4424, 1:2,000). Green, isotype control; red, sorting nexin 5.
Immunocytochemical staining of HAP1 cells with anti-Sorting nexin 5 antibody (Cat#4424, 1:1,000). Nuclei were stained blue with DAPI;Sorting nexin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Scale bar, 20 μm.
Western blotting analysis using anti-sorting nexin 5 antibody (Cat#4424). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sorting nexin 5 antibody (Cat#4424, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of sorting nexin 5 expression in HAP-1 cells using anti-sorting nexin 5 antibody (Cat#4424, 1:2,000). Green, isotype control; red, sorting nexin 5.
Immunocytochemical staining of HAP1 cells with anti-Sorting nexin 5 antibody (Cat#4424, 1:1,000). Nuclei were stained blue with DAPI;Sorting nexin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Scale bar, 20 μm.
Western blotting analysis using anti-sorting nexin 5 antibody (Cat#4424). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sorting nexin 5 antibody (Cat#4424, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of sorting nexin 5 expression in HAP-1 cells using anti-sorting nexin 5 antibody (Cat#4424, 1:2,000). Green, isotype control; red, sorting nexin 5.
Immunocytochemical staining of HAP1 cells with anti-Sorting nexin 5 antibody (Cat#4424, 1:1,000). Nuclei were stained blue with DAPI;Sorting nexin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Scale bar, 20 μm.
Western blotting analysis using anti-sorting nexin 5 antibody (Cat#4424). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sorting nexin 5 antibody (Cat#4424, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
