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Anti-APBB1 Rabbit Monoclonal Antibody #4545

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Flow cytometric analysis of APBB1 expression in HAP-1 cells using anti-APBB1 antibody (Cat#4545, 1:2,000). Green, isotype control; red, APBB1.

Immunocytochemical staining of HAP-1 cells with anti-APBB1 antibody (Cat#4545, 1:1,000) . Nuclei were stained blue with DAPI; APBB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-APBB1 antibody (Cat#4545). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APBB1 antibody (Cat#4545, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

APBB1
货号：

4545
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-APBB1 Rabbit Monoclonal Antibody
Aliases	APBB1; Amyloid Beta Precursor Protein Binding Family B Member 1; FE65; RIR; Amyloid Beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65); Amyloid-Beta A4 Precursor Protein-Binding Family B Member 1; Adaptor Protein FE65a2; Stat-Like Protein; Protein Fe65; MGC:9072; Fe65
Background	Gene Name: APBB1 NCBI Gene Entry: 322 UniProt Entry: O00213
Application Information	Molecular Weight: Predicted, 77 kDa; observed, 100 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11755 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human FE65
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of APBB1 expression in HAP-1 cells using anti-APBB1 antibody (Cat#4545, 1:2,000). Green, isotype control; red, APBB1.
Immunocytochemical staining of HAP-1 cells with anti-APBB1 antibody (Cat#4545, 1:1,000) . Nuclei were stained blue with DAPI; APBB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-APBB1 antibody (Cat#4545). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APBB1 antibody (Cat#4545, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

APBB1
货号：

4545
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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