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Anti-PSMA1 Rabbit Monoclonal Antibody #4565

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Flow cytometric analysis of PSMA1 expression in HT-1080 cells using anti-PSMA1 antibody (Cat#4565, 1:2,000). Green, isotype control; red, PSMA1.

Immunocytochemical staining of HT-1080 cells with anti-PSMA1 antibody (Cat#4565, 1:1,000). Nuclei were stained blue with DAPI; PSMA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PSMA1 antibody (Cat#4565). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMA1 antibody (Cat#4565, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PSMA1
货号：

4565
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-PSMA1 Rabbit Monoclonal Antibody
Aliases	Proteasome 20S Subunit Alpha 1; PROS30; HC2; NU; Proteasome (Prosome, Macropain)Subunit,Alpha Type,1; Multicatalytic Endopeptidase Complex Subunit C2; Proteasome Subunit Alpha Type-1; Proteasome Subunit Alpha 1; 30 KDa Prosomal Protein; Proteasome Component C2;Macropain Subunit C2; Proteasome Nu Chain; MGC14542; MGC14575; MGC14751
Background	Gene Name: PSMA1 NCBI Gene Entry: 5682 UniProt Entry: P25786
Application Information	Molecular Weight: Predicted, 30 kDa; observed, 30 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11850 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Proteasome 20S C2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PSMA1 expression in HT-1080 cells using anti-PSMA1 antibody (Cat#4565, 1:2,000). Green, isotype control; red, PSMA1.
Immunocytochemical staining of HT-1080 cells with anti-PSMA1 antibody (Cat#4565, 1:1,000). Nuclei were stained blue with DAPI; PSMA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PSMA1 antibody (Cat#4565). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMA1 antibody (Cat#4565, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PSMA1
货号：

4565
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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