Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody #4270

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Flow cytometric analysis of phospho-GSK3 beta (Ser9) expression in HepG2 cells using anti-phospho-GSK3 beta (Ser9) antibody (Cat#4270, 1:2,000). Green, isotype control; red, phospho-GSK3 beta (Ser9).

Immunocytochemical staining of HepG2 cells with anti-Phospho-GSK3 beta (Ser9) antibody (Cat#4270, 1:1,000). Nuclei were stained blue with DAPI; Phospho-GSK3 beta (Ser9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-phospho-GSK3 β (Ser9) antibody (Cat#4270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-GSK3 β (Ser9) antibody (Cat#4270, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

GSK3B
货号：

4270
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody
Aliases	GSK3B; Glycogen Synthase Kinase 3 Beta; Serine/Threonine-Protein Kinase GSK3B; Glycogen Synthase Kinase-3 Beta; EC 2.7.11.26; GSK-3 Beta; EC 2.7.11.1; EC 2.7.11
Background	Gene Name: GSK3B NCBI Gene Entry: 2932 UniProt Entry: P49841
Application Information	Molecular Weight: Predicted, 47 kDa; observed, 46 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10380 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-GSK3 beta (Ser9)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of phospho-GSK3 beta (Ser9) expression in HepG2 cells using anti-phospho-GSK3 beta (Ser9) antibody (Cat#4270, 1:2,000). Green, isotype control; red, phospho-GSK3 beta (Ser9).
Immunocytochemical staining of HepG2 cells with anti-Phospho-GSK3 beta (Ser9) antibody (Cat#4270, 1:1,000). Nuclei were stained blue with DAPI; Phospho-GSK3 beta (Ser9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-GSK3 β (Ser9) antibody (Cat#4270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-GSK3 β (Ser9) antibody (Cat#4270, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

GSK3B
货号：

4270
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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