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Anti-UBE2M Rabbit Monoclonal Antibody #5540

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Flow cytometric analysis of UBE2M expression in HepG2 cells using anti-UBE2M antibody (Cat#5540, 1:2,000). Green, isotype control; red, UBE2M.

Immunocytochemical staining of HepG2 cells with anti-UBE2M antibody (Cat#5540, 1:1,000) . Nuclei were stained blue with DAPI; UBE2M was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-UBE2M antibody (Cat#5540). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2M antibody (Cat#5540, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

UBE2M
货号：

5540
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-UBE2M Rabbit Monoclonal Antibody
Aliases	UBE2M; Ubiquitin Conjugating Enzyme E2 M; BC12; HUbc12; Ubiquitin-Conjugating Enzyme E2M (UBC12 Homolog, Yeast); NEDD8-Conjugating Enzyme Ubc12; NEDD8 Carrier Protein; Ubiquitin-Conjugating Enzyme E2M (Homologous To Yeast UBC12); Epididymis Secretory Sperm Binding Protein; Ubiquitin-Conjugating Enzyme E2 M; Ubiquitin-Conjugating Enzyme E2M; Ubiquitin Conjugating Enzyme E2M; Ubiquitin Carrier Protein M; Ubiquitin-Protein Ligase M; NEDD8 Protein Ligase; Yeast UBC12 Homolog; EC 2.3.2.34; EC 6.3.2; UBC-RS2
Background	Gene Name: UBE2M NCBI Gene Entry: 9040 UniProt Entry: P61081
Application Information	Molecular Weight: Predicted, 21 kDa; observed, 21 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB1980 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human UBE2M
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of UBE2M expression in HepG2 cells using anti-UBE2M antibody (Cat#5540, 1:2,000). Green, isotype control; red, UBE2M.
Immunocytochemical staining of HepG2 cells with anti-UBE2M antibody (Cat#5540, 1:1,000) . Nuclei were stained blue with DAPI; UBE2M was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-UBE2M antibody (Cat#5540). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2M antibody (Cat#5540, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

UBE2M
货号：

5540
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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