Immunohistochemical analysis of Cytokeratin 18 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Cytokeratin 18 expression in HEK293T (A), EC9706 (B), SGC7901 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen
Recombinant protein of human Cytokeratin 18
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.3) containing 50% glycerol, and 0.05% Proclin300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:1,000 Immunohistochemistry (IHC): 1:100-1:200
Note
This product is for research use only.
Picture
Immunohistochemical analysis of Cytokeratin 18 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Cytokeratin 18 expression in HEK293T (A), EC9706 (B), SGC7901 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.
Immunohistochemical analysis of Cytokeratin 18 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Cytokeratin 18 expression in HEK293T (A), EC9706 (B), SGC7901 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.
