Western blotting analysis using anti-beta-actin antibody (Cat#5177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta-actin antibody (Cat#5177, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human beta-Actin
Isotype
Mouse IgG2b
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.05% Proclin 300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:4,000-1:20,000
Note
This product is for research use only.
Data
Western blotting analysis using anti-beta-actin antibody (Cat#5177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta-actin antibody (Cat#5177, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-beta-actin antibody (Cat#5177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta-actin antibody (Cat#5177, 1:20,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
