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Anti-Smad2 Rabbit Monoclonal Antibody #7512

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Flow cytometric analysis of Smad2 expression in HepG2 cells using anti-Smad2 antibody (Cat#7512,1:2,000). Green, isotype control; red, Smad2.

Immunocytochemical staining of HepG2 cells with Smad2 antibody (Cat#7512, 1:1,000). Nuclei were stained blue with DAPI; Smad2 as stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Smad2 antibody (Cat#7512). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smad2 antibody (Cat#7512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SMAD2
货号：

7512
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Smad2 Rabbit Monoclonal Antibody
Aliases	SMAD2; SMAD Family Member 2; JV18-1; MADR2; MADH2; Mothers Against Decapentaplegic Homolog 2; MAD Homolog 2; HMAD-2; HSMAD2; MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2; Sma- And Mad-Related Protein 2; Mothers Against DPP Homolog 2; Mother Against DPP Homolog 2; Mad-Related Protein 2; SMAD 2; CHTD8; Smad2; JV18; LDS6
Background	Gene Name: SMAD2 NCBI Gene Entry: 4087 UniProt Entry: Q15796
Application Information	Molecular Weight: Predicted, 52 kDa; observed, 58 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB965 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Smad2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Smad2 expression in HepG2 cells using anti-Smad2 antibody (Cat#7512,1:2,000). Green, isotype control; red, Smad2.
Immunocytochemical staining of HepG2 cells with Smad2 antibody (Cat#7512, 1:1,000). Nuclei were stained blue with DAPI; Smad2 as stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Smad2 antibody (Cat#7512). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smad2 antibody (Cat#7512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SMAD2
货号：

7512
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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