Flow cytometric analysis of CCAR2 expression in HT-1080 cells using anti-CCAR2 antibody (Cat#5189, 1:2,000). Green, isotype control; red, CCAR2.
Immunocytochemical staining of HT-1080 cells with anti-CCAR2 antibody (Cat#5189, 1:1,000) . Nuclei were stained blue with DAPI; CCAR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
Western blotting analysis using anti-CCAR2 antibody (Cat#5189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCAR2 antibody (Cat#5189, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
CCAR2; Cell Cycle And Apoptosis Regulator 2; KIAA1967; DBC-1; NET35; DBC1; Cell Division Cycle And Apoptosis Regulator Protein; Cell Cycle And Apoptosis Regulator Protein; Deleted In Breast Cancer 1; P30 DBC; Deleted In Breast Cancer Gene 1 Protein; DBIRD Complex Subunit KIAA1967; P30DBC; DBC.1
Flow cytometric analysis of CCAR2 expression in HT-1080 cells using anti-CCAR2 antibody (Cat#5189, 1:2,000). Green, isotype control; red, CCAR2.
Immunocytochemical staining of HT-1080 cells with anti-CCAR2 antibody (Cat#5189, 1:1,000) . Nuclei were stained blue with DAPI; CCAR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
Western blotting analysis using anti-CCAR2 antibody (Cat#5189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCAR2 antibody (Cat#5189, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CCAR2 expression in HT-1080 cells using anti-CCAR2 antibody (Cat#5189, 1:2,000). Green, isotype control; red, CCAR2.
Immunocytochemical staining of HT-1080 cells with anti-CCAR2 antibody (Cat#5189, 1:1,000) . Nuclei were stained blue with DAPI; CCAR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
Western blotting analysis using anti-CCAR2 antibody (Cat#5189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCAR2 antibody (Cat#5189, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
