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Anti-SCD Rabbit Monoclonal Antibody #5218

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Flow cytometric analysis of Stearoyl-CoA desaturase expression in HepG2 cells using anti-Stearoyl-CoA desaturase antibody (Cat#5218, 1:2,000). Green, isotype control; red, Stearoyl-CoA desaturase.
Immunocytochemical staining of HepG2 cells with anti-Stearoyl-CoA desaturase antibody (Cat#5218, 1:1,000) . Nuclei were stained blue with DAPI; Stearoyl-CoA desaturase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-stearoyl-CoA desaturase antibody (Cat#5218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-stearoyl-CoA desaturase antibody (Cat#5218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    SCD
  • 货号：
    5218
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-SCD Rabbit Monoclonal Antibody
Aliases SCD; Stearoyl-CoA Desaturase; FADS5; SCD1; Fatty Acid Desaturase; Acyl-CoA Desaturase; SCDOS; Stearoyl-CoA Desaturase (Delta-9-Desaturase); Stearoyl-CoA Desaturase Opposite Strand; Delta(9)-Desaturase; EC 1.14.19.1; HSCD1; Predicted Protein Of HQ0998; Delta-9-Desaturase; Delta-9 Desaturase; MSTP008
Background

Gene Name: SCD

NCBI Gene Entry: 6319

UniProt Entry: O00767
Application Information

Molecular Weight: Predicted, 42 kDa; observed, 25-37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB215

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human SCD1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:1,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Stearoyl-CoA desaturase expression in HepG2 cells using anti-Stearoyl-CoA desaturase antibody (Cat#5218, 1:2,000). Green, isotype control; red, Stearoyl-CoA desaturase.
  • Immunocytochemical staining of HepG2 cells with anti-Stearoyl-CoA desaturase antibody (Cat#5218, 1:1,000) . Nuclei were stained blue with DAPI; Stearoyl-CoA desaturase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-stearoyl-CoA desaturase antibody (Cat#5218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-stearoyl-CoA desaturase antibody (Cat#5218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    SCD
  • 货号：
    5218
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Stearoyl-CoA desaturase expression in HepG2 cells using anti-Stearoyl-CoA desaturase antibody (Cat#5218, 1:2,000). Green, isotype control; red, Stearoyl-CoA desaturase.
Immunocytochemical staining of HepG2 cells with anti-Stearoyl-CoA desaturase antibody (Cat#5218, 1:1,000) . Nuclei were stained blue with DAPI; Stearoyl-CoA desaturase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-stearoyl-CoA desaturase antibody (Cat#5218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-stearoyl-CoA desaturase antibody (Cat#5218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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