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Anti-PPP1R12A Rabbit Polyclonal Antibody #52586

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Immunohistochemical analysis of PPP1R12A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of PPP1R12A expression in HEK293T (A), A2780 (B), C6 (C), SP20 (D) whole cell lysates.
  • 基因名:
    PPP1R12A
  • 货号:
    52586
  • 应用:
    WB, IHC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-PPP1R12A Rabbit Polyclonal Antibody
Aliases PPP1R12A; Protein Phosphatase 1 Regulatory Subunit 12A; MBS; Myosin Phosphatase-Targeting Subunit 1; MYPT1; M130; Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 12A; Protein Phosphatase Myosin-Binding Subunit; Myosin Binding Subunit; Protein Phosphatase 1, Regulatory Subunit 12A; Myosin Phosphatase, Target Subunit 1; Myosin Phosphatase Target Subunit 1; GUBS
Background

Gene Name: PPP1R12A

NCBI Gene Entry: 4659

UniProt Entry: O14974

Application Information

Molecular Weight: Predicted, 115 kDa; observed, 140 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human PPP1R12A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of PPP1R12A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of PPP1R12A expression in HEK293T (A), A2780 (B), C6 (C), SP20 (D) whole cell lysates.
Citation(0)
Immunohistochemical analysis of PPP1R12A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of PPP1R12A expression in HEK293T (A), A2780 (B), C6 (C), SP20 (D) whole cell lysates.
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