Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cathepsin A antibody (Cat#52712, 1:400) at 4°C, followed by operating according to SP Kit instructionsand DAB staining.
Western blotting analysis using anti-Cathepsin A antibody (Cat#52712). Total lysates (25 μg) were loaded and separated by SDS-PAGE.The blot was incubated with anti-Cathepsin A antibody (Cat#52712, 1:500) and conjugated goat anti-rabbit secondary antibody respectively.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human Protective protein
Isotype
Rabbit IgG
Storage Buffer
Supplied in TBS (pH 7.4) containing 50% glycerol, 1% BSA and 0.02% Proclin 300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry (IHC): 1:100-1:500 Immunocytochemistry (IC): 1:100-1:500
Note
This product is for research use only.
Picture
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cathepsin A antibody (Cat#52712, 1:400) at 4°C, followed by operating according to SP Kit instructionsand DAB staining.
Western blotting analysis using anti-Cathepsin A antibody (Cat#52712). Total lysates (25 μg) were loaded and separated by SDS-PAGE.The blot was incubated with anti-Cathepsin A antibody (Cat#52712, 1:500) and conjugated goat anti-rabbit secondary antibody respectively.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cathepsin A antibody (Cat#52712, 1:400) at 4°C, followed by operating according to SP Kit instructionsand DAB staining.
Western blotting analysis using anti-Cathepsin A antibody (Cat#52712). Total lysates (25 μg) were loaded and separated by SDS-PAGE.The blot was incubated with anti-Cathepsin A antibody (Cat#52712, 1:500) and conjugated goat anti-rabbit secondary antibody respectively.
