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Anti-Annexin A1 Rabbit Monoclonal Antibody #5366

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Flow cytometric analysis of Annexin A1 expression in HepG2 cells using anti-Annexin A1 antibody (Cat#5366, 1:2,000). Green, isotype control; red, Annexin A1.
Immunocytochemical staining of HepG2 cells with anti-Annexin A1 antibody (Cat#5366, 1:1,000). Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-annexin A1 antibody (Cat#5366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A1 antibody (Cat#5366, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    ANXA1
  • 货号:
    5366
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Annexin A1 Rabbit Monoclonal Antibody
Aliases ANXA1; Annexin A1; ANX1; LPC1; Phospholipase A2 Inhibitory Protein; Chromobindin-9; Calpactin II; Calpactin-2; Annexin-1; Epididymis Secretory Sperm Binding Protein; Annexin I (Lipocortin I); Lipocortin I; Annexin I; P35
Background

Gene Name: ANXA1

NCBI Gene Entry: 301

UniProt Entry: P04083

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1160

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human Annexin A1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Annexin A1 expression in HepG2 cells using anti-Annexin A1 antibody (Cat#5366, 1:2,000). Green, isotype control; red, Annexin A1.
  • Immunocytochemical staining of HepG2 cells with anti-Annexin A1 antibody (Cat#5366, 1:1,000). Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-annexin A1 antibody (Cat#5366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A1 antibody (Cat#5366, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of Annexin A1 expression in HepG2 cells using anti-Annexin A1 antibody (Cat#5366, 1:2,000). Green, isotype control; red, Annexin A1.
Immunocytochemical staining of HepG2 cells with anti-Annexin A1 antibody (Cat#5366, 1:1,000). Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-annexin A1 antibody (Cat#5366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A1 antibody (Cat#5366, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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