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Anti-FERMT2 Mouse Monoclonal Antibody #5421

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Flow cytometric analysis of FERMT2 expression in HepG2 cells using anti-FERMT2 antibody (Cat#5421, 1:2,000). Green, isotype control; red, FERMT2.
Immunocytochemical staining of HepG2 cells with anti-FERMT2 antibody (Cat#5421, 1:1,000). Nuclei were stained blue with DAPI; FERMT2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-FERMT2 antibody (Cat#5421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FERMT2 antibody (Cat#5421, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    FERMT2
  • 货号:
    5421
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-FERMT2 Mouse Monoclonal Antibody
Aliases FERMT2; FERM Domain Containing Kindlin 2; KIND2; UNC112B; PLEKHC1; Mig-2; Pleckstrin Homology Domain Containing, Family C (With FERM Domain) Member 1; PH Domain-Containing Family C Member 1; Fermitin Family Homolog 2; Mitogen Inducible Gene-2; Fermitin Family Member 2; Kindlin-2; MIG2; Pleckstrin Homology Domain Containing, Family C Member 1; Pleckstrin Homology Domain-Containing Family C Member 1; Fermitin Family Homolog 2 (Drosophila); Mitogen Inducible Gene 2 Protein; Mitogen-Inducible Gene 2 Protein; Kindlin 2; UNC112; MIG-2
Background

Gene Name: FERMT2

NCBI Gene Entry: 10979

UniProt Entry: Q96AC1

Application Information

Molecular Weight: Predicted, 78 kDa; observed, 73 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1435

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human FERMT2
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of FERMT2 expression in HepG2 cells using anti-FERMT2 antibody (Cat#5421, 1:2,000). Green, isotype control; red, FERMT2.
  • Immunocytochemical staining of HepG2 cells with anti-FERMT2 antibody (Cat#5421, 1:1,000). Nuclei were stained blue with DAPI; FERMT2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-FERMT2 antibody (Cat#5421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FERMT2 antibody (Cat#5421, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of FERMT2 expression in HepG2 cells using anti-FERMT2 antibody (Cat#5421, 1:2,000). Green, isotype control; red, FERMT2.
Immunocytochemical staining of HepG2 cells with anti-FERMT2 antibody (Cat#5421, 1:1,000). Nuclei were stained blue with DAPI; FERMT2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-FERMT2 antibody (Cat#5421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FERMT2 antibody (Cat#5421, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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