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Anti-GRIA1 Rabbit Monoclonal Antibody #5432

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Flow cytometric analysis of GRIA1 expression in HepG2 cells using anti-GRIA1 antibody (Cat#5432, 1:2,000). Green, isotype control; red, GRIA1.

Immunocytochemical staining of HepG2 cells with anti-GRIA1 antibody (Cat#5432, 1:1,000) . Nuclei were stained blue with DAPI; GRIA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-GRIA1 antibody (Cat#5432). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GRIA1 antibody (Cat#5432, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

GRIA1
货号：

5432
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-GRIA1 Rabbit Monoclonal Antibody
Aliases	GRIA1; Glutamate Ionotropic Receptor AMPA Type Subunit 1; Glutamate Receptor 1; GluA1; GLURA; GLUR1; Glutamate Receptor, Ionotropic, AMPA 1; AMPA-Selective Glutamate Receptor 1; AMPA Receptor Subunit GluA1; GluR-K1; GluR-1; GluR-A; GLUH1; Glutamate Receptor Ionotropic, AMPA 1; AMPA 1; HBGR1; MRD67; MRT76; GLUA1
Background	Gene Name: GRIA1 NCBI Gene Entry: 2890 UniProt Entry: P42261
Application Information	Molecular Weight: Predicted, 102 kDa; observed, 130 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB1525 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human GRIA1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of GRIA1 expression in HepG2 cells using anti-GRIA1 antibody (Cat#5432, 1:2,000). Green, isotype control; red, GRIA1.
Immunocytochemical staining of HepG2 cells with anti-GRIA1 antibody (Cat#5432, 1:1,000) . Nuclei were stained blue with DAPI; GRIA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-GRIA1 antibody (Cat#5432). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GRIA1 antibody (Cat#5432, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

GRIA1
货号：

5432
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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