Flow cytometric analysis of ACAP2 expression in HT-1080 cells using ACAP2 antibody (Cat#61116, 1:2,000). Green, isotype control; red, ACAP2.
Immunocytochemical staining of HT-1080 cells with ACAP2 antibody (Cat#61116, 1:1,000). Nuclei were stained blue with DAPI; ACAP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-ACAP2 antibody (Cat#61116). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACAP2 antibody (Cat#61116, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ACAP2, ArfGAP with coiled-coil, ankyrin repeat and PH domains 2.
Western blotting analysis using anti-ACAP2 antibody (Cat#61116). ACAP2 expression in wild type (WT) and ACAP2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ACAP2 antibody (Cat#61116, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of ACAP2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ACAP2 antibody (Cat#61116, 1:2,000) and analyzed using BD flow cytometer.
