Flow cytometric analysis of MGEA5 expression in HAP-1 cells using MGEA5 antibody (Cat#61204, 1:2,000). Green, isotype control; red, MGEA5.
Immunocytochemical staining of HAP-1 cells with MGEA5 antibody (Cat#61204, 1:1,000). Nuclei were stained blue with DAPI; MGEA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MGEA5 antibody (Cat#61204). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MGEA5 antibody (Cat#61204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-MGEA5 antibody (Cat#61204). MGEA5 expression in wild-type (WT) and OGA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MGEA5 antibody (Cat#61204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). OGA, O-GlcNAcase.
