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KD-Validated Anti-MAP2K1 Rabbit Monoclonal Antibody #61296

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Flow cytometric analysis of MAP2K1 expression in HepG2 cells using MAP2K1 antibody (Cat#61296, 1:2,000). Green, isotype control; red, MAP2K1.

Immunocytochemical staining of HepG2 cells with MAP2K1 antibody (Cat#61296, 1:1,000). Nuclei were stained blue with DAPI; MAP2K1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-MAP2K1 antibody (Cat#61296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP2K1 antibody (Cat#61296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-MAP2K1 antibody (Cat#61296). MAP2K1 expression in wild type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAP2K1 antibody (Cat#61296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

MAP2K1
货号：

61296
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-MAP2K1 Rabbit Monoclonal Antibody
Aliases	MAP2K1; Mitogen-Activated Protein Kinase Kinase 1; MEK1; Dual Specificity Mitogen-Activated Protein Kinase Kinase 1; MAPK/ERK Kinase 1; MAPKK1; PRKMK1; MKK1; ERK Activator Kinase 1; MAP Kinase Kinase 1; EC 2.7.12.2; MAPKK 1; MEK 1; Protein Kinase, Mitogen-Activated, Kinase 1 (MAP Kinase Kinase 1); CFC3; MEL
Background	Gene Name: MAP2K1 NCBI Gene Entry: 5604 UniProt Entry: Q02750
Application Information	Molecular Weight: Predicted, 43 kDa, observed, 45 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2940 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human MEK1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of MAP2K1 expression in HepG2 cells using MAP2K1 antibody (Cat#61296, 1:2,000). Green, isotype control; red, MAP2K1.
Immunocytochemical staining of HepG2 cells with MAP2K1 antibody (Cat#61296, 1:1,000). Nuclei were stained blue with DAPI; MAP2K1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-MAP2K1 antibody (Cat#61296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP2K1 antibody (Cat#61296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-MAP2K1 antibody (Cat#61296). MAP2K1 expression in wild type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAP2K1 antibody (Cat#61296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

MAP2K1
货号：

61296
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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