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KD-Validated Anti-DDR2 Rabbit Monoclonal Antibody #61314

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Immunocytochemical staining of HT-1080 cells with DDR2 antibody (Cat#61314, 1:1,000). Nuclei were stained blue with DAPI; DDR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
Western blotting analysis using anti-DDR2 antibody (Cat#61314). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDR2 antibody (Cat#61314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-DDR2 antibody (Cat#61314). DDR2 expression in wild-type (WT) and DDR2 shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DDR2 antibody (Cat#61314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    DDR2
  • 货号:
    61314
  • 应用:
    WB, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-DDR2 Rabbit Monoclonal Antibody
Aliases DDR2; Discoidin Domain Receptor Tyrosine Kinase 2; TKT; NTRKR3; TYRO10; Discoidin Domain-Containing Receptor Tyrosine Kinase 2; Discoidin Domain Receptor Family, Member 2; Discoidin Domain-Containing Receptor 2; Receptor Protein-Tyrosine Kinase TKT; CD167 Antigen-Like Family Member B; Tyrosine-Protein Kinase TYRO10; Discoidin Domain Receptor 2; EC 2.7.10.1; Neurotrophic Tyrosine Kinase, Receptor-Related; Neurotrophic Tyrosine Kinase Receptor Related 3; Cell Migration-Inducing Protein 20; Migration-Inducing Gene 16 Protein; Hydroxyaryl-Protein Kinase; CD167b Antigen; EC 2.7.10; MIG20a; WRCN
Background

Gene Name: DDR2

NCBI Gene Entry: 4921

UniProt Entry: Q16832

Application Information

Molecular Weight: Predicted, 97 kDa; observed, 97 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2210

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human DDR2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HT-1080 cells with DDR2 antibody (Cat#61314, 1:1,000). Nuclei were stained blue with DAPI; DDR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
  • Western blotting analysis using anti-DDR2 antibody (Cat#61314). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDR2 antibody (Cat#61314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-DDR2 antibody (Cat#61314). DDR2 expression in wild-type (WT) and DDR2 shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DDR2 antibody (Cat#61314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Immunocytochemical staining of HT-1080 cells with DDR2 antibody (Cat#61314, 1:1,000). Nuclei were stained blue with DAPI; DDR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
Western blotting analysis using anti-DDR2 antibody (Cat#61314). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDR2 antibody (Cat#61314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-DDR2 antibody (Cat#61314). DDR2 expression in wild-type (WT) and DDR2 shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DDR2 antibody (Cat#61314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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