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KD-Validated Anti-CIRBP Rabbit Monoclonal Antibody #61328

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Flow cytometric analysis of CIRBP expression in HepG2 cells using CIRBP antibody (Cat#61328, 1:2,000). Green, isotype control; red, CIRBP.

Immunocytochemical staining of HepG2 cells with CIRBP antibody (Cat#61328, 1:1,000). Nuclei were stained blue with DAPI; CIRBP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-CIRBP antibody (Cat#61328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CIRBP antibody (Cat#61328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CIRBP, cold inducible RNA binding protein.

Western blotting analysis using anti-CIRBP antibody (Cat#61328). CIRBP expression in wild type (WT) and CIRBP shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CIRBP antibody (Cat#61328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CIRBP
货号：

61328
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-CIRBP Rabbit Monoclonal Antibody
Aliases	CIRBP; Cold Inducible RNA Binding Protein; CIRP; Cold-Inducible RNA-Binding Protein; Glycine-Rich RNA Binding Protein; A18 HnRNP; Glycine-Rich RNA-Binding Protein CIRP; Cold Inducible RNA-Binding Protein; Testicular Tissue Protein Li 39; A18HNRNP
Background	Gene Name: CIRBP NCBI Gene Entry: 1153 UniProt Entry: Q14011
Application Information	Molecular Weight: Predicted, 19 kDa, observed, 19 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB7980 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from CIRBP
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CIRBP expression in HepG2 cells using CIRBP antibody (Cat#61328, 1:2,000). Green, isotype control; red, CIRBP.
Immunocytochemical staining of HepG2 cells with CIRBP antibody (Cat#61328, 1:1,000). Nuclei were stained blue with DAPI; CIRBP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-CIRBP antibody (Cat#61328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CIRBP antibody (Cat#61328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CIRBP, cold inducible RNA binding protein.
Western blotting analysis using anti-CIRBP antibody (Cat#61328). CIRBP expression in wild type (WT) and CIRBP shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CIRBP antibody (Cat#61328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CIRBP
货号：

61328
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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