Flow cytometric analysis of Calcium modulating ligand expression in HeLa cells using anti-Calcium modulating ligand antibody (Cat#63803, 1:1,000). Green, isotype control; red, Calcium modulating ligand.
Immunocytochemical staining of Hela cells with anti-Calcium modulating ligand associated protein antibody (Cat#63803, 1:1,000) . Nuclei were stained blue with DAPI; Calcium modulating ligand associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-calcium modulating ligand antibody (Cat#63803). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calcium modulating ligand antibody (Cat#63803, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-calcium modulating ligand antibody (Cat#63803). Calcium modulating ligand expression in wild type (WT) and calcium modulating ligand (CAMLG) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-calcium modulating ligand antibody (Cat#63803, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
