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KD-Validated Anti-ALAS1 Rabbit Monoclonal Antibody #61368

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Flow cytometric analysis of ALAS1 expression in HAP-1 cells using anti-ALAS1 antibody (Cat#61368, 1:2,000). Green, isotype control; red, ALAS1.

Immunocytochemical staining of HAP-1 cells with anti-ALAS1 antibody (Cat#61368, 1:1,000) . Nuclei were stained blue with DAPI; ALAS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-ALAS1 antibody (Cat#61368). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALAS1 antibody (Cat#61368, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-ALAS1 antibody (Cat#61368). ALAS1 expression in wild type (WT) and ALAS1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ALAS1 antibody (Cat#61368, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ALAS1
货号：

61368
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-ALAS1 Rabbit Monoclonal Antibody
Aliases	ALAS1; 5'-Aminolevulinate Synthase 1; ALAS-H; ALAS3; 5-Aminolevulinate Synthase, Non-Specific, Mitochondrial; Aminolevulinate, Delta-, Synthase 1; 5-Aminolevulinic Acid Synthase 1; Delta-Aminolevulinate Synthase 1; Delta-ALA Synthase 1; EC 2.3.1.37; ALASH; ALAS; 5-Aminolevulinate Synthase, Nonspecific, Mitochondrial; Migration-Inducing Protein 4; EC 2.3.1; MIG4
Background	Gene Name: ALAS1 NCBI Gene Entry: 211 UniProt Entry: P13196
Application Information	Molecular Weight: Predicted, 71 kDa, observed, 71 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3190 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Alas1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ALAS1 expression in HAP-1 cells using anti-ALAS1 antibody (Cat#61368, 1:2,000). Green, isotype control; red, ALAS1.
Immunocytochemical staining of HAP-1 cells with anti-ALAS1 antibody (Cat#61368, 1:1,000) . Nuclei were stained blue with DAPI; ALAS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ALAS1 antibody (Cat#61368). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALAS1 antibody (Cat#61368, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ALAS1 antibody (Cat#61368). ALAS1 expression in wild type (WT) and ALAS1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ALAS1 antibody (Cat#61368, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ALAS1
货号：

61368
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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